Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Validated RP-HPLC method for the quantification of aprepitant in bulk and pharmaceutical dosage forms
Author(s): K. Krishna Chaitanya, D. Gowri Sankar, D. Samson Israel and Ch. Narasimha kumarA reverse phase HPLC method has been developed and validated for the estimation of Aprepitant in Pharmaceutical dosage forms. An X-Terra RP -8, 150x4.6 mm, 5 μm particle size, with mobile phase consisting of Buffer (weigh and transfer 0.34g of Tetra-n butyl ammonium Sulphate in 1000ml of purified water, Add 1.1ml of concentrated Ortho phosphoric acid (88%) Mix properly) and Acetonitrile in the ratio of 50:50% v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 4.86 min. The detector response was linear in the concentration of 25-150 μg/ml. The respective linear regression equation being y = 22769x – 1489 (R² = 0.999). The limit of detection and limit of quantification was 0.28 and 0.84 μg/ml respectively. The percentage assay of Aprepitant capsules was found to be 100.02 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Aprepitant in bulk drug and in its pharmaceutical dosage form.
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