The diversity of bacteria in blood cultures of susp
ected patients with typhoid 
fever based on fenetics numerical analysis and resp
onse to antibiotics in 
Jayapura, Papua

Andi Hajrah; 1
 Tri Gunaedi; 2
 Daniel Lantang
2; Yohanis Ngili
3

Abstract

The diversity of bacteria in blood cultures of susp ected patients with typhoid fever based on fenetics numerical analysis and resp onse to antibiotics in Jayapura, Papua

Author(s): Andi Hajrah, 1 Tri Gunaedi, 2 Daniel Lantang 2 and Yohanis Ngili 3

Suspected patients with typhoid fever is diagnosed patients and physical examination found symptoms of fever, gastrointestinal disorders and disorders of conscio usness with clinical syndromes of typhoid incomplet e so it needs to be supported by laboratory description that show s typhoid, including bacteriological examination of blood cultures and serologic widal. Bacteremia-septicemia is a complication that causes typhoid fever bacter ia in the blood. Research using laboratory experimental metho d is implemented in health laboratory of Jayapura, Papua Province, with reference checks Test Widal obtained 135 patients in the city of Jayapura. Widal Test p ositive in 88 samples (65.2%) were found 81 samples (92.0%) patie nts with suspected typhoid fever has no bacterial g rowth (sterile), 7 samples (8.0%) grew gram-positive cocc i (57.1%) and bacterial Gram-negative bacilli (42.9 %). Based on fenetics numerical analysis using the Vitek 2 co mpact and test of sensitivity to 14 kinds of antibi otics, bacteria are obtained diversity of Gram-negative bacilli as much as 3 isolates; Escherecia coli, Pseodomonas ae ruginosa, Pseudomonas maltophila and 4 isolates of gram-posit ive cocci such as Staphylococcus aureus, Staphyloco ccus epidermidis, Staphylococcus hominis and Staphylococ cus saprophyticus. Dendogram of fenetics structure numerical analysis results, obtained 6 clusters with similari ty between 56.6% - 97.0% use reference strains of S . aureus subspecies aureus ATCC 25923 and P. aeruginosa ATCC 27853. Sensitivity of 7 isolates were found agains t 14 types of antibiotics obtained Nitrofurantoin and Ge ntamycin have high sensitivity (100%), while Trimet hoprim - Sulfamethoxazole has decreased sensitivity of 57.1% and 92.3% (12/13) of sensitive antibiotics against Gram positive cocci and 63.6% (7/11) of antibiotic-resis tant bacteria Gram-negative bacilli

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