Insecticidal activity of essential oil and non-volatile fraction from the stem of wild zingiberaceae Amomum apiculatum against Drosophila melanogaster have been assayed. The assay consists of mortality, antifeedant, and repellent. Essential oil concentrations varied 0 (as control) ; 0,1; 0,5; 1%. The assay result showed that the higher percentage for mortality, antifeedant, and repellent are 35,33; 96,61; 73,33 % respectively. The concentrations of non-volatile fraction also varied 0; 0,1; 0,5 %. The assay result showed that the higher percentage for mortality, antifeedant, and repellent for methanol crude extract are 17,80; 95,02; 26,67 % respectively. For n-hexane fraction the higher percentage for mortality, antifeedant, and repellent are 42,20; 96,03; 80 % respectively. For ethyl acetate fraction the higher percentage for mortality, antifeedant, and repellent are 60; 96,35; 73,33 % respectively. The essential oil and non-volatile fraction from the stem of Amomum apiculatum also inhibit regeneration process of Drosophila melanogaster. The assay result showed that in the 5th day of assay eggs and larva appears in control medium, but eggs and larva did not appear in medium of essential oil and non-volatile fraction. This result conclude that the essential oil and non-volatile fraction from the stem of Amomum apiculatum had the potential as natural insecticide.
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