Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Spectrophotometric estimation and validation of hydrochlorothiazide in tablet dosage forms by using different solvents
Author(s): Hapse. S. A, Wagh. V. S, Kadaskar. P. T, Dokhe. M. D. and Shirsath. A. SThe method for the estimation of hydrochlorothiazide in tablet dosage form has been developed. This analytical method developed for the estimation of hydrochlorothiazide in bulk fluids showed maximum absorbance at λmax of 272 nm in distilled water and in 0.01N NAOH between 200 nm and 400 nm of UV scan. Linearity studies indicated that estimation of hydrochlorothiazide between 5.00μg /ml to 30.00 μg /ml in distilled water and 1.00μg/ml to 30.00μg/ml in 0.01N NAOH were found to be linear with regression equation of y = 0.043x+0.198; ( r2 = 0.999) and y=0.059+0.029;(r2 =0.998). The method developed was validated for accuracy, linearity, limit of detection and limit of quantitation studies. The above analytical parameters indicated that the developed UV Spectrophotometric method of hydrochlorothiazide was simple, accurate and reproducible. Also the biggest advantage is of using distilled water and 0.01N NAOH as solvent for the first time saving organic solvent exposure and also stable solvent is less expensive.
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Citations : 15261
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