Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Simultaneous identification of caffeine????¸ acetaminophen, sildenafilcitrate, tadalafil and vardenafil HCl in aphrodisiac traditional herbal medicines by Thin Layer Chromatography-Densitometry
Author(s): Reni Septiani and Sophi DamayantiThin Layer Chromatography (TLC)-Densitometry has been developed and validated for the identification of caffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl in traditional medicine aphrodisiac simultaneously. Liquid-liquid extraction method was used for sample preparation and then continued by TLCDensitometry using TLC-Scanner-4 CAMAG. The method used silica gel 60 F254 as the stationary phase. Mobile Phase for TLC was ethyl acetate, n-propanol, NH3 25%(45:5:1). Rf values ofcaffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl and tadalafil were 0.57, 0.69, 0.27, 0.77 and 0.42, respectively. Resolution value (R) of caffeine-acetaminophen, acetaminophen-tadalafil, sildenafil citrate-vardenafil HCl, and vardenafil HCl-caffeine was obtained in a value of 2.0, 1.5, 2.4 and 2.4, respectively. This method have limits of detection (LOD) of reference standards for caffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl were 0.1475, 0.2273, 0.1346, 0.1951 and 0.2047 ïÂÂ????g/g spot whereas LOD of spiking were 0.3350, 0.3817, 0.1716, 0.3031 and 0.2659 ïÂÂ????g/g spot,respectively.
Select your language of interest to view the total content in your interested language
Google Scholar citation report
Citations : 15261
Der Pharma Chemica received 15261 citations as per Google Scholar report
Der Pharma Chemica peer review process verified at publons
