Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Setting up and Calibration of Simultaneous Dual Color Wide Field Microscope for Single Molecule Imaging
Author(s): Diaa Atta, Aly Okasha and Medhat IbrahimSingle molecule spectroscopy is considered among the most important spectroscopic and microscopic analytical tools especially that follow the biological reactions. The usage of wide field microscope in single molecule spectroscopy is not something new but what we introduce is a simultaneous dual color imaging wide field microscope setup. The setup alignments tested, and all the species we used to prepare the sample were tested free from unwanted fluorescence. A real single molecule experiment was done using streptavidin stained with Atto655.The system is proved to be able to detect single molecules in two channels exited with different colors at the same time, and it is ready for the experiments.
Select your language of interest to view the total content in your interested language
Google Scholar citation report
Citations : 15261
Der Pharma Chemica received 15261 citations as per Google Scholar report
Der Pharma Chemica peer review process verified at publons
