Screening for in vitro Antifungal activity and Qualitative Phytochemical
analysis of the root extract of Jasminum angustifolium

Tharmarajah Manoranjan; Arulanantham C. Thavaranjit; Shathyaa Kathirkamanathan

Abstract

Screening for in vitro Antifungal activity and Qualitative Phytochemical analysis of the root extract of Jasminum angustifolium

Author(s): Tharmarajah Manoranjan, Arulanantham C. Thavaranjit and Shathyaa Kathirkamanathan

Jasminum angustifolium is a species of Jasmine endemic to Sri Lanka. Roots and leaves are of medicinal value. Roots are bitter, acrid and are useful for external application in ring worm and herpes and are recommended for ophthalmopathy, ulcerative stomatitis, leprosy, pruritus and wounds. Present study was carried out to evaluate the in vitro antifungal activity of sequentially extracted different solvent extracts of roots of Jasminum angustifolium against plant pathogenic fungi such as Aspergillus spp., Penicillium spp., Trichoderma spp., Fusarium spp. and Alternaria spp. by the standard agar well diffusion method. Synthetic fungicide Dithane M-45 (Mancozeb) and solvents used for extraction as standard and controls respectively. Qualitative phytochemical analysis of crude extracts was also carried out for the presence of bioactive compounds using standard procedures. Results showed that the antifungal activity was exhibited by three solvents root extracts of J. angustifolium against all fungi except hexane and methanol root extracts failed to inhibit the growth of Aspergillus spp .and Trichoderma spp. respectively. Growth of Penicillium spp.(15mm) was significantly inhibited by all three root extracts than compared to other fungi tested. Growth inhibition by hexane and ethylacetate root extracts was significantly higher than compared to methanol root extract on the growth of Fusarium spp.(12mm,13mm) and Alternaria spp.(12mm,11mm). Ethylacetate root extract of J. angustifolium showed a significant effect on fungal growth inhibition compared with the other two root extracts. Different types of phytochemicals were present in sequentially extracted hexane, ethylacetate and methanol root extracts. Tannins, saponins, phlobatanins and steroids were present but flavanoids and terpenoids were absent in all three extracts.

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