Abstract

Screening and HPLC quantitation of phytocompounds with antiobesity activity in traditional teas consumed in Zimbabwe

Author(s): Dzomba P., L. Gwatidzo, K. Mtwazi and M. Mupa

Obesity is now a major problem, leading to life threatening complications such as hypertension and diabetes type II. This study was focused on screening and quantifying phytocompounds portraying α-amylase inhibitory activity in selected traditional herbal teasfor possible use as ethnobotanical therapeutics for management of obesity. Phytocompounds were extracted using liquid-solid and solid phase extraction using C18 cartridges followed by a separating stage using Thin Liquid Chromatography. Quantitation of antiobesity phytochemicals was achieved using reversed phase HPLC. The three herbal teas that is Myrothamnus flabellifolius, Lippia javanica and Ficus sur were all found to consist of flavonoids.In addition L. javanica extract consisted of alkaloids, saponins and terpenoids while M. flabellifolius extract consisted of saponins and alkaloids. F. sur also consisted of alkaloids. Flavonoids from all the three plants showed antiobesity activity while alkaloids failed to inhibit α-amylase. M. flabellifolius flavonoids exhibited the highest percentage inhibition of α-amylase activity of 88 %, followed by L. javanica at 83 % and lastly F. sur at 81 %. The concentration of flavonoids in M. flabellifolius was found to be 256.92 ± 4.41 mg/mL while in L. javanica was 125.60 ± 2.16 mg/mL.F. sur exhibited the lowest flavonoid content of 118.61 ± 2.04 mg/mL. The present research shows that dietary flavonoids from the three herbal teas can be used as ethnobotanical therapeutics for management of obesity.

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