Quantitative estimation of (+)-Catechin in stem bark of Saraca asoka Linn using HPTLC

Permender Rathee; Sushila Rathee; Deepti Rathee; Dharmender Rathee

Abstract

Quantitative estimation of (+)-Catechin in stem bark of Saraca asoka Linn using HPTLC

Author(s): Permender Rathee, Sushila Rathee, Deepti Rathee, Dharmender Rathee

Saraca asoka is an important plant of Indian system of medicine for its chemical constituents and its well known pharmacological activities. Catechin is a well-known flavonoid found in this plant and also in many foods plants and often utilized by naturopaths for the symptomatic treatment of several gastrointestinal, respiratory and vascular diseases. Our aim was to quantify this flavonoid by using HPTLC from Saraca asoca stem bark. In the present paper we report our work on quantification of this marker compound by thin layer chromatography densitometric methods using high performance thin layer chromatography. To the best of our knowledge, this is the first report of quantification of this compound using HPTLC from this plant. The thin layer chromatography densitometric methods were found to be precise with RSD for intra-day in the range of 0.35-2.5 and for inter-day in the range of 0.85-3.05 for different concentrations of Catechin. Instrumental precision was 3.36 (% RSD) for Catechin. Accuracy of the method was checked by conducting recovery studies at three different levels for the compound and the average percentage recoveries obtained was 101.45%. Saraca asoca sample was found to contain 0.048% w/w of Catechin.

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