Abstract

Protective Effect of Brassica olearacea Var. Capitata Extract on Glutathione-s-transferase, Catalase and Total Protein Activity in Dolutegravir-induced Toxicity

Author(s): Amagon L*, Falang KD, Bukar BB, Wanche EM and Amagon KI

Introduction: Oxidative stress is one of the leading causes of liver injury; the process of such damage involves the molecular reactions of highly reactive oxygen species that can lead to impaired liver functions.

Objective: This study investigated the protective effect of Brassica oleracea (Red Cabbage against Dolutegravir-induced oxidative stress and assessed the safety of the plant extract in Drosophila melanogaster.

Methods: In the first phase, there were three groups of flies in replicates of five (5), with each vial contained 50 flies of both gender; group one was the control, and the other flies were administered the toxicant for ten days, then homogenized, and the biochemical assays performed. In the second phase, there were five groups of flies in replicates of five (5), each vial containing 50 flies. They were administered the extract for seven days, exposed to the toxicant for Five (5) days, and homogenized. The biochemical assay was carried out to determine the level of glutathione-s-transferase and catalase.

Results: Brassica oleracea extract was observed to be safe in the flies exposed to the extract. Glutathione-s-transferase and catalase activity was reduced in the group treated with dolutegravir but increased in the presence of different concentrations of Brassica oleracea.

Conclusion: The extract of Brassica oleracea showed a good ability to protect against dolutegravir-induced toxicity and was also safe in D. melanogaster.

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