Abstract

Phenotypic Diversity of BmNPV Isolated from Different Geographical Locations of South India: A Biophysical Study

Author(s): Madhuri Sathyanarayana*, S. E. Neelagund, Dayanand B. Sajjan and Rajat Hegde

Viral diseases take the lives of n number of silkworms each year resulting in 30%-40% of average crop loss. Grasserie is one of the deadly diseases caused by NPV (Nuclear Polyhedrosis Virus) for silkworm. BmNPV was observed throughout the year with increased severity in the summer and autumn crops. Morphological parameters of occlusion bodies of BmNPV from different strains were examined to validate in detail structure of these viruses. This examination mainly focused on light microscopic and electron microscopic study along with size and surface charge determination using SZ-100 nanoparticle series instrument. Resulted in intelligible images of different isolates under various magnifications. Further, the average OB size was found to be 150.7 nm, 138.8 nm, 143.8 nm, 140.0 nm and 140.5 nm in diameter for HRH, HSR, KNK, MSI and VKK BmNPV strain respectively. The zeta potential is often measured as a function of pH or other change in the chemistry to help formulators create new products with a long shelf life. The zeta-potentials of isolates were found to be more of negative because of the presence of PEP with the charge of -29.0 mV, -35.4 mV, -45.0 mV, -28.1 mV and -23.5 mV for HRH, HSR, KNK, MSI and VKK BmNPV strain respectively. Thus, by uncovering these aspects may help to spell out indetail phenotypic diversity of BmNPV isolates from South India.

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