Abstract

Nano Entomopathogenic Fungi As biological Control Agents on Cabbage Worm, Pieris rapae L. (Lepidoptera: Pieridae)

Author(s): Abdel-Raheem M. A, Naglaa F. Reyad, Al-Shuraym Laila. A. and Abdel-Rahman I. E.

The present investigation was carried out during two successive Cabbage seasons (2014-2015 and 2015- 2016), to study the impact of entomopathogenic fungi on Cabbage worm, Pieris rapae L. The Pieris rapae Populations were evaluated in the field early in the season in October 2014.which began to appear on cabbage plants. Thereafter number of Pieris rapae increased gradually to reach a peak of abundance during November 2014 and November 2015. ). Three concentrations were used (1 x103, 1 x 104 and 1 x 105 spores/ ml.). Under laboratory conditions the results showed that B. bassiana, M. anisopliae and V. lecanii have a latent toxicity because mortalities were occurred after the third day from treatment. The maximum percent of mortality (100 %) was occurred after the tenth day from treatment with the third concentration in V. lecanii. The Second concentration (1 x 104spores/ ml.) was the highly toxic in B. bassiana, M. anisopliae and V. lecanii to the larvae of Pieris rapae compared with the other two concentrations. Under field conditions the third concentration (1 x 105) also, was the best concentration against Pieris rapae L. after the third application in B. bassiana, M. anisopliae and V. lecanii. The percent of reduction was ranged between 60 and 70% in all concentrations. B. bassiana and M. anisopliae were high effective than V. lecanii against Pieris rapae L. These results confirmed that B. Bassiana, M. anisopliae and V. lecanii isolates are promising agents for Pieris rapae L. control in the field.

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