Method validation of ninhydrin-based spectrophotome
try for trace analysis of 
cyanide residue in an antiseptic alcohol

Piriyaporn Pilachai; Teerut Baosanthia; Saksit C
hanthai

Abstract

Method validation of ninhydrin-based spectrophotome try for trace analysis of cyanide residue in an antiseptic alcohol

Author(s): Piriyaporn Pilachai, Teerut Baosanthia and Saksit C hanthai*

In this study, ultratrace level of cyanide was dete cted based on the catalytic reaction of ninhydrin i n the presence of cyanide ion under the optimum conditions. The absor bance of the blue solution of 2-cyano-1,2,3-trihydr oxy-2H indene was measured at both maximum wavelengths of 352 and 598 nm. The developed method gave linear ra nge of 0.02-0.1 μ g/mL for cyanide ion. Effect of various kinds of al cohols on the absorbance was investigated. Generall y, cyanide is a toxic substance with LC 50 at μ g/mL, which is rather difficult to determine. Thus, this method was adopted for higher accuracy and precision. At prese nt, alcohols produced from cyanide-rich cassava are commercially available which could be contaminated with the cyanide residue. In this case, an antisept ic alcohol, isopropanol 70% (v/v), was subject as a model solut ion for cyanide testing with satisfied recovery and without any interference from the diluted isopropanol solution

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