Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Method Development and Validation For the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Tablet Formulation by HPTLC
Author(s): A. R. Magesh and M. D. DhanarajuHigh performance thin layer chromatographic (HPTLC) method has been developed and validated for simultaneous investigation of Irbesartan and Hydrochlorothiazide in tablet formulation. Chromatographic separation was performed on aluminium plates precoated with silica gel 60F254, with methanol: ethyl acetate (7: 3 % v/v) as mobile phase. Detection was performed densitometrically at 254 nm. The Rf values of Irbesartan and Hydrochlorothiazide were 0.24 ± 0.10and 0.42 ± 0.06, respectively. Linearity was found to be in the concentration range of 150-900 ng/spot for Irbesartan and 25- 150 ng/spot for Hydrochlorothiazide, accuracy (100.05 % for Irbesartan and 100.26 % for Hydrochlorothiazide) and specificity, in accordance with ICH guidelines. The method can be used for routine analysis of Irbesartan and Hydrochlorothiazide in tablet formulation.
Select your language of interest to view the total content in your interested language
Google Scholar citation report
Citations : 15261
Der Pharma Chemica received 15261 citations as per Google Scholar report
Der Pharma Chemica peer review process verified at publons
