Abstract

Liquid chromatography-mass spectrometry (LC-MS) method for the determination of sugars in fresh pomegranate fruit juices

Author(s): Mahmood Salman, El-Sayed S. Abdel-Hameed, Salih A. Bazaid, Musbah G. Al-Shamrani, Hanaa F. Mohamed

Pomegranate is an excellent source of many nutrients able to contribute to a healthy diet. Taif is the first leading Pomegranate producers in Saudi Arabia. To our knowledge, there has been no research on the Taif Pomegranate fruit juices. In this study, qualitative and quantitative identification of sugars (fructose, glucose and sucrose) was carried out for four different pomegranates origins commercially available in Taif province, KSA such as (Abha, Egyptian, Yamani 1, Yamani 2) using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) technique. The LC-MS method optimized in term of the kind of column used mobile phase, and the flow rate, in addition to the parameters for mass spectrometry. The results showed that the concentrations of fructose and glucose in the analyzed samples were between (0.162-0.306 gm/100 gm) and (0.132-0.300 gm/100 gm) respectively. The sucrose was not detected. In conclusion, the authors recommend these methods for analysis of sugars concerning the food and human health safety.

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