This work was carried out of 200 samples from Abo-Ghalab- Kafr El-Shikh Governorate and Elnobaria - Elbehira Governorate only 30 samples (15 %) contained Entomopathogenic fungi, i.e., 18 isolates from Beauveria bassiana, 12 isolates from Metarhizium anisopliae. The fungi were propagated on Peptone media and rice grains. Peptone media gave the highest conidiospore production by B. bassiana and the rice media gave the highest conidiospore by M. anisopliae. Conidia were prepared in laboratory for bioassay at the concentrations of 2 x 10 2, 2 x 10 3 and 2 x 10 4 spores/ ml. (C1, C2 & C3). Results obtained successful control against larvae of Galleria mellonella. Studies were carried out in the laboratory. The entomopathogenic fungi showed a high effect on Pegomyia mixta either in the laboratory and field experiments.
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