For the first time, complete isolation and identification of fungal isolate has been performed in the realm of eco-friendly process for synthesis of nanomaterials. The isolated Aspergillus niger G3-1 has been used to bring about Silver Nanoparticles (AgNPs) and their influence on the antibacterial property of cotton fabrics based textiles. The results obtained from UV-Vis analysis showed the Surface Plasmon Resonance (SPR) absorption at 400 nm. Transmission Electron Microscopy (TEM) revealed that the spherical biogenic AgNPs were found to be between 5 and 26 nm in size. Fourier Transform Infrared Spectroscopy (FTIR) band at 1639.24 cm-1 referred to the binding vibrations of amide I band of protein with N-H stretching band, indicating possible interaction between silver and proteins. These evidences suggest that the release of extracellular protein molecules and possibly perform the function of the formation and stabilization of AgNPs in living cell filtrate of A. niger G3-1. Moreover, the particle size analyzer proved that, the size of the as- formed AgNPs was polydispersed mixture which 98.4% of the main size diameter lies around 64.87 nm. Finally, AgNPs were applied to cotton fabrics which impart antibacterial activity to the cotton fabric with growth reduction reached 88-95% towards Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis
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