In this study the effect of different concentrations of three gas mixture (carbon dioxide, nitrogen,oxygen) ,and also vacuum conditions and flexible multi-layer films has been studied on sensory evaluation of smoked kutum fish (Rutilus frisii kutum) at ambient condition (T= 25 0C) . Ordinary condition as control packaging were compared with four types of modified atmosphere packaging: (N270%+ CO230%), (N230% + CO270%), (45%CO2+45%N2+10%O2), and vacuum conditions, in this project. Smoked kutum fish were packaged in 3 kinds of flexible multi-layer containers under modified atmosphere packaging,{3-layers(PET(12)/AL(12)/LLD(100)), 4- layers (PET(12)/AL(7)/PET(12)/LLD(100) ),and 3- layer( PET(12)/AL(7)/LLD(100) }. Samples were performed sensory evaluation, in different times, with 15 treatment ,3 run, statistical analysis and comparison of data, were done by software SAS (Ver:9/1) and Duncan’s new multiple range test, with confidence level of 95% (P <0.05). The shelf life of smoked kutum fish according to organoleptic properties were reported under conditions 1,2,3 in 4-layers , 60,58,45 days and in vacuum conditions were about 40 days, in 3-layers (AL:12) , under conditions 1,2, 3 and vacuum conditions were 55,50,40,35 days, with 3-layers(AL:7),under conditions1,2,3 were 45,40,35 days and in vacuum conditions were 30 days. During the period of this experiment (60 days) sensory properties of samples in various conditions, had significant differences. Sensory evaluation showed that increasing CO2 concentration extended shelf life, and could affected texture,color, appearance ,smell, taste of smoked kutum fish. Especially taste & texture had better result. under gas composition (N230%+ CO270%) with 4- layers , since steam permeability of 4-layer was less than 3-layer According to these results could be concluded the best condition for sensory evaluation belonged to treatment under modified atmosphere CO2 70% with flexible pouch {PET (12)/AL(7)/PET(12)/LLD(100)} .
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