Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Effect of internal standard on HPLC analysis of tablet dosage forms: An experimental study with statistical comparison
Author(s): Mustafa Çelebier, Merve Nenni, Ayşegül Doğan, Ä°ncilay Süslü and Sacide AltınözThe purpose of this study was to investigate the effect of internal standard on precision and accuracy of an HPLC method for the analysis of tablet dosage forms. Paracetamol was chosen as a model molecule and caffeine was used as an internal standard for this purpose. An experimental procedure was applied and a statistical comparison was performed to present the effect of IS on precision and accuracy for the quality control analysis. IS was added in a constant amount (5, 20 and 40 μg mL-1) into the samples and calibration curve and internal standard techniques were used to determine the amount of PA in tablet dosage forms. As it is known, using IS corrects the loss of analyte during sample preparation or sample inlet. The results for calibration curve and internal standard techniques were statistically evaluated and discussed. According to the results in our experimental conditions, the internal standard technique statistically affected the analysis results but did not improve the precision and accuracy
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Citations : 15261
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