Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry
ISSN: 0975-413X
Abstract
Development and validation of high performance liquid chromatographic method for the Simultaneous Estimation of Candesartan cilexetil and Hydrochlorothiazide in combined tablet dosage form
Author(s): K. Balamuralikrishna and B. SyamasundarA simple, sensitive and reproducible reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of Candesartan cilexetil and Hydrochlorothiazide in combined tablet dosage form. Chromatography was performed on a 250 mm x 4.6 mm, 5-μm particle size, C8 Hypersil BDS column with a 60:40 (v/v) mixture of acetonitrile and Triethylamine (0.02 %) as a mobile phase and the pH was adjusted to 5.5 with dilute o-phosphoric acid. The detection of the combined dosage form was carried out at 262 nm and a flow rate employed was 1.0 mL/min. The retention times were 2.449 and 4.895 min for Candesartan and Hydrochlorothiazide respectively. Linearity was obtained in the concentration range 50 to 150 μg/mL for Candesartan cilexetil and 75-225 μg/mL for Hydrochlorothiazide, with a correlation coefficient of 0.9999 and 0.9999. The results of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method.
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Citations : 15261
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