GET THE APP

Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Hydrochlorothiazide and Irbesartan | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Hydrochlorothiazide and Irbesartan

Author(s): Aniruddha R. Chabukswar, Swati C. Jagdale, Bhanudas S. Kuchekar, Pradeep D Lokhande, Santosh N. Shinde, Kunal D. Ingale, Anuja K. Kolsure

A simple, precise, rapid and accurate RP-HPLC-PDA method has been developed for the simultaneous estimation of Hydrochlorothiazide and Irbesartan in tablet formulations. The chromatographic separation was achieved on Waters Symmetry C18 column (250 mm x 4.6 mm, 5.0 μ particle size) using methanol: THF: acetate buffer (60:10:30v/v) pH adjusted to 5.5 with acetic acid, flow rate was 0.75 ml/min and column was maintained at 500C. Quantification and linearity was achieved at 271 nm over the concentration range of 0.3 – 50 μg /ml for Hydrochlorothiazide and 3.6 - 600 μg/ml for Irbesartan. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness. The proposed method was optimized and validated as per the ICH guidelines.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 15261

Der Pharma Chemica received 15261 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry