GET THE APP

Determination methadone in hair using directly suspended droplet three phase microextraction technique with UV-visible spectroscopy | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Determination methadone in hair using directly suspended droplet three phase microextraction technique with UV-visible spectroscopy

Author(s): Malihe Samadi Kazemi and Somayeh Alizadeh

In this study, we are used Liquid–liquid–liquid microextraction (LLLME) with directly suspended droplet using uvvisible spectroscopy for extraction and determination of methadone in hair. The target compound was extracted from the aqueous sample solution (donor phase, pH 9) into an organic phase and then was back-extracted into a directly suspended droplet of an acidic aqueous solution (acceptor phase, pH 1). In this method, without using a microsyringe as supporting device, an aqueous large droplet is freely suspended at the top-center position of an immiscible organic solvent, which is laid over the aqueous sample solution while being agitated. Then, the droplet was withdrawn into the microsyringe and determined with UV detection at λ=205 nm. 1-octhanol (350μL) used as organic phase. The results show extraction time: 15 min, back-extraction time: 5 min and stirring rate: 600 rpm. Also, these conditions used for determination methadone in hair samples of abusers were collected from 13 men ranging from 16 to 45 years old. The calibration graph was linear in the range of 0.2 -10 μg mL-1 with r = 0.995(N=5). The limit of detection and the enrichment factor were 1.7 ng mL-1 and 159.92, respectively. The relative recovery was in the range of 82–98% with an average of 91.50%. Also the relative standard deviation (RSD, n = 5) obtained 4.3%. All experiments were carried out at room temperature (25±0.5 °C).


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 15261

Der Pharma Chemica received 15261 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry