Natural substances from plants have multiple interests put to use in the industry. Essential oils are a very interesting group of these metabolites that feature different properties that make them interesting. We did this study to obtain an extract essential oil from the plant Mentha pepirita South region Algerian cultivated to the Municipality of Ouargla. the Mint, widespread in Algeria, of the family Lamiaceae is a native herb of several therapeutic properties (antiseptic, anti-neuralgic, analgesic ...). The extraction of its aromatic fraction offers new perspectives in aromatherapy by hydrodistillation method by a Clevenger-type apparatus. The extract obtained was the subject of Antibacterial and antifungal activity and a chemical study includes determining the chemical essential oil composition by GC-MS which showed the product exsistance which might present different interests. The Antibacterial and antifungal activity of oils was tested using the direct contact method against the growth of different Fusarium. The results showed great potential of natural antimicrobial activity against strains tested. The analysis of this oil by GC-MS, lets notice the presence of several components which (23.3%), Followed by 7- Oxabicyclo[4,1,0]heptane,1-methyl-4-(methylethenyl)- (14,6%), Cis-(-)-1,2-Epoxy-p-menth-8-ene (5,72%), and Bicyclo[2.2.1] heptane-2,5-diol,1,7,7-trimethyl-,(2-endo,5-exo)- (4,04%) are the major components.
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