In the present study, evaluation of antibacterial activity of Acetic Acid extracts of medicinal plant Limoniastrum guyonianum from Ouargla and El Oued regions (Algeria) against three bacteria strains: Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) was done by using disc agar diffusion method. Acetic Acid extracts showed antibacterial activity against all the tested strains. Diameter inhibition values ranged in extracts of L. guyonianum Ouargla (LO) from 15 mm to 24.3333 mm, while diameter inhibition values ranged in extract of L. guyonianum El Oued (LE) from 12 mm to 24.3333 mm.
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