Development and Validation of Spectrophotometric Method for Determination of Ceftriaxone Sodium in Pharmaceutical Dosage Forms
By K. R. Patel, V. D. Patel, K. P. Patel, V. G. Patel
Three new, simple, sensitive, selective and economical methods (Method 1, 2 and 3) for the determination of Ceftriaxone Sodium have been described in this paper. Method 1 and 2 are based on the formation of coloured Schiff base obtained when Ceftriaxone Sodium in acidic conditions related p-dimethyleaminobenzaldihyde and salicyldihyde in ethanol to form yellow coloured chromogens exhibiting lmax at 410 nm and 390 nm respectively. Method 3 is based on coupling the drug with 3-methyle-2-benzothiazolinone hydrazonehydrochloride in the presence of ferric chloride to form blue coloured chromogen exhibiting lmax at 628 nm. These method obeyed Beer’s law in the concentration range of 10-50 mg/ml, 20-100 mg/ml and 2-10 mg/ml respectively. The results for analysis for the three methods have been validated statistically and by recovery studies. The results are comparable with those obtained with UV spectrophotometric method in double distilled water at lmax 241 nm.
Keyword: Ceftriaxone Sodium, p-dimethyleaminobenzaldihyde, salicylaldehyde, Optical Characteristics.