Quantification of β- Sitosterol using HPTLC from Capparis decidua (Forsk.) Edgew
By Sushila Rathee, O. P. Mogla, Permender Rathee and Dharmender Rathee
Capparis decidua (Gudhapatra) is traditionally used for curing a variety of ailments such as toothache, cough, asthma, intermittent fever and rheumatism in India. There are no reports of quantification of β- Sitosterol from this plant. Hence a TLC densitometric method has been developed and validated for quantification of this marker compound. β-Sitosterol was quantified from methanolic extract using the Solvent System of Toluene: Methanol (9: 1, v/v). The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for β-Sitosterol was 80–480 ng/spot and its content was 0.0244 % w/w. This simple, precise and accurate method gave good resolution from other constituents of extract. It can be adopted for routine quality control of herbal material and formulations containing Capparis decidua.
Key words: Capparis decidua; HPTLC; β- Sitosterol; Densitometric method.