Synthesis and invitro spleen cell proliferative activity of novel oxime derivative of Ibuprofenamide
By Neeraj Mehta, Suresh Thareja, Saurabh Agarwal, Priyanka Malla, Tilak Raj Bhardwaj and Manoj Kumar
Many chronic inflammatory reactions are characterized by tissue infiltration of activated T-lymphocytes. The immunosuppressant activity of NSAIDs correlates with their ability to block transcription factors required for the expression of inducible response genes triggered by T-cell antigen receptor (TCR) following encounter with antigens including NF-KB, NF-AT and activated protein 1.The present work explores mechanism of NSAIDs involved in macrophage and T-cell mediated inflammatory response. Oxime derivative of ibuprofenamide was synthesized and evaluated for T-cell and B-cell proliferation activity (MTT assay) at concentrations ranging from 10-1000 μg/well. Results indicate suppression of the T-cell proliferation and stimulation of B-cell mitogen LPS.
Key words: Inflammation, MTT, NSAID, Oxime, TCR.