Quantitative Estimation of Gallic Acid in Triphala Churnam Tablet by RP-HPLC
By Babu Ganesan, Perumal Perumal, VijayaBaskaran Manickam, Suribabu Mummudi, Srujana Divya Gotteti, Surya Rao Srikakolapu, Latha Sundaresan Thirumurthy
A RP-HPLC assay method has been developed and validated for the estimation of gallic acid. Chromatography was carried on C18 column (250 mm x 4.6 mm I.D., 5 μm) by isocratic elution utilizing a mobile phase of acetonitrile and water containing 0.01 % v/v ortho phosphoric acid (in the ratio of 80: 20% v/v) with UV detection at wavelength 270 nm at the flow rate 1mL/min. The proposed method was validated for sensitivity, specificity, linearity, accuracy, precision, ruggedness, robustness and solution stability. The response of the drug was linear in the concentration range of 20-120 μg/mL. Limit of detection and limit of quantification was found to be 6.13 µg/mL and 18.57 µg/mL respectively. The % recovery ranged within 98-102%. Method, system, interday and intraday precision were also found to be within the limits of acceptance criteria. Method was found to be rugged when analysis was carried out by different analyst. The proposed method is rapid, simple and also it can be applied for the routine analysis of herbal formulations.
Keywords : Gallic acid, Triphala churnam tablets, RP-HPLC.