In-vitro antioxidant potential of various extracts of whole plant of Bridelia scandens (Roxb) wild
By D. Senthil Kumar, A. Kottai Muthu, D. Satheesh Kumar, R. Manavalan
The present investigation was to examine the in-vitro antioxidant potential of various extracts of whole plant of Bridelia scandens by different in-vitro methods. The antioxidant activity was determined by Hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity with reference standard Ascorbate and total phenol content respectively. An IC50 value was found that methanolic extract of Bridelia scandens is more effective in hydroxyl radical scavenging activity than that of petroleum ether and ethyl acetate extracts. The methanolic extract of Bridelia scandens was found to more effective in the nitric oxide radical scavenging activity. The IC50 values of the methanolic extract of Bridelia scandens and ascorbate were found to be 130µg/ml and 410µg/ml respectively. But when compare to the all the three extracts with ascorbate (standard), the methanolic extract of the Bridelia scandens showed the better result. In addition, the methanolic extract of Bridelia scandens was found to contain a noticeable amount of total phenols, which play a major role in controlling antioxidants. So, the in-vitro studies clearly showed that the methanolic extract of Bridelia scandens has a significant antioxidant activity. These in-vitro assays indicate that this plant extracts is a better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.
Keywords : Whole plant of Bridelia scandens, In-vitro antioxidant, Hydroxyl radical scavenging activity, Nitric oxide radical activity, Total Phenol.