Micellar Liquid Chromatographic Method Development for Determination of Rosuvastatin Calcium and Ezetimibe in Pharmaceutical Combination Dosage Form
By Smita Sharma, M. C. Sharma, D. V. Kohli, S. C. Chaturvedi
This paper described validated, rapid, simple and sensitive liquid chromatographic procedure that use micellar mobile phase containing only Tween-20 and n-Butanol, is reported for the determination of method for estimation of Rosuvastatin Calcium (ROS) and Ezetimibe (EZE) in tablet dosage form. HPLC separation was achieved on Licrosphere C18 column (250 x 4.6mm) using Tween-20 and n-Butanol Phosphate buffer, pH 5.1 (60:20:20 v/v) at flow rate of 1.0 ml/min at 25oC temperature. Quantitation was achieved by UV detection at 314 nm over the concentration range 5-10 mg/ml for both the drugs with mean recoveries of 99.91% + 0.12 and 100.11% + 0.29 for ROS and EZE respectively. This method is simple, precise and sensitive and it is applicable for the simultaneous estimation of ROS and EZE in tablet dosage form.
Keywords : Rosuvastatin Calcium, Ezetimibe Micellar liquid chromatography, Tween-20