Simultaneous analysis of Paracetamol and Tramadol - Analytical method development & validation
By Sridhar Narayan, Pradeep Kumar, Rakesh K Sindhu, Ayushman Tiwari, Manik Ghosh
In the present study the development and validation of analytical method was carried out for simultaneous analysis of paracetamol and tramadol using first derivative method. The λ max was determined for paracetamol and tramadol and standard curves were plotted. The stability of the stock solutions with due course of time was also determined and compared using t-Test. Further the simultaneous analysis at 1:1, 8:1 and 1:8 ratios revealed the need of a higher derivative method for the simultaneous estimation of paracetamol and tramadol in various dosage forms.
Keywords : Paracetamol, tramadol, simultaneous estimation, method development, validation